beta Catenin (CTNNB1) Rabbit Polyclonal Antibody

CAT#: AP02722PU-S

beta Catenin (CTNNB1) rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 1,400.00


货期*
2周

规格
    • 50 ul

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Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry on paraffin sections: 1/50 - 1/100.
Immunofluorescence: 1/100 - 1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence around aa. 35~39 (I-H-S-G-A) derived from Human b-Catenin.
Specificity beta-Catenin antibody detects endogenous levels of total beta-Catenin protein.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State: Aff - Purified
State: Liquid purified IgG fraction
Concentration lot specific
Purification Affinity chromatography
Conjugation Unconjugated
Storage Condition Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name catenin beta 1
Background Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Synonyms CTNNB1, CTNNB, Beta-catenin
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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