MSK1 (RPS6KA5) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Suitable for use in in Western blot (1:500-1:1000).
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized non-phosphopeptide derived from human MSK1 around the phosphorylation site of serine 376 (G-Y-Sp-F-V).
Specificity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. MSK1 antibody AP02710PU detects endogenous levels of total MSK1 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	ribosomal protein S6 kinase A5
Database Link	Entrez Gene 9252 Human UniProt ID O75582
Background	MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain.
Synonyms	Ribosomal protein S6 kinase alpha-5, RSKL
Reference Data

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