IKB alpha (NFKBIA) Rabbit Polyclonal Antibody

CAT#: AP02651PU-S

IKB alpha (NFKBIA) rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 1,400.00


货期*
2周

规格
    • 50 ul

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Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry on paraffin sections: 1/50 -1/100.
Immunofluorescence: 1/100 - 1/200.
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic non-phosphopeptide derived from human IkappaB-alpha around the phosphorylation site of serine 32/36 (H-D-S-G-L-D-S-M-K).
Specificity IkappaB-alpha antibody detects endogenous levels of total IkappaB-alpha protein.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Immunoaffinity chromatography
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name NFKB inhibitor alpha
Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IkappaB alpha and IkappaB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkappaB and block nuclear import by masking the nuclear localisation sequences of NFkappaB. The activation of NFkappaB involves the inducible phosphorylation and subsequent degradation of IkappaB. Immunoblotting easily detects the hyperphosphorylated forms of IkappaB alpha, but not phosphorylated IkappaB beta. Interestingly, IkappaB alpha and IkappaB beta mediate different NFkappaB responses. IkappaB alpha appears to control more transient activation of NFkappaB in response to an inducer, while IkappaB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkappaB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkappaB is confirmed by immunoblotting.
Synonyms I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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