IGF1 Receptor (IGF1R) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on Paraffin Embedded Tissue: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence aa. 1159-1163 (D-I-Y-E-T) derived from human IGF-1R.
Specificity	The antibody detects endogenous levels of total IGF-1R protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	Predicted MW: 200 95 kd
Gene Name	insulin like growth factor 1 receptor
Database Link	Entrez Gene 3480 Human UniProt ID P08069
Background	There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Synonyms	IGF-I receptor, IGF1 Receptor
Reference Data

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