STAT3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Incubate membrane with diluent antibody in 5% nonfat milk, 1xTBS, 0.1% tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Syntehtic peptide KLH conjugated corresponding to sequence around amino acids 725~729 (P-M-SP-P-R) derived from Human STAT3 protein.
Specificity	This antibody detects endogenous levels of total STAT3 protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity-Chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	signal transducer and activator of transcription 3
Database Link	Entrez Gene 6774 Human UniProt ID P40763
Background	STAT3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. STAT3 is also constitutively activated in a number of human tumors, possesses oncogenic potential, and anti-apoptotic activities. Following phosphorylation at Y705, STAT3 dimerizes, translocates to the nucleus and binds DNA. Transcriptional activation seems to be regulated by phosphorylation at Ser 727. STAT3 exists as an alpha form (86kD) and a beta form (79kD) that lacks a 50 amino acid domain containing Ser 727.
Synonyms	STAT-3, Acute-phase response factor, APRF
Note	Molecular Weight: 88 kDa
Reference Data

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