JUND Rabbit Polyclonal Antibody

CAT#: AP02570PU-N

JUND rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 2,250.00


货期*
2周

规格
    • 100 ul

Product images

Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry on paraffin sections: 1/50 - 1/100.
Immunofluorescence: 1/100 - 1/200.
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of serine 255 (G-E-Sp-P-P).
Specificity JunD antibody detects endogenous levels of total JunD protein.
Formulation PBS( without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol
State: Aff - Purified
State: Liquid purified IgG fraction
Concentration lot specific
Purification Affinity-chromatography using epitope-specific immunogen
Conjugation Unconjugated
Storage Condition Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Gene Name JunD proto-oncogene, AP-1 transcription factor subunit
Background JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.
Synonyms JUND
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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