NFkB p100 / p52 (NFKB2) Rabbit Polyclonal Antibody

CAT#: AP02558PU-N

NFkB p100 / p52 (NFKB2) rabbit polyclonal antibody, Aff - Purified

Size: 50 ul 100 ul



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CNY 2,250.00


货期*
2周

规格
    • 100 ul

Cited in 2 publications.

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Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Immunofluorescence: 1/100 - 1/200.
Immunohistochemistry on Paraffin Sections: 1/50 - 1/100.
Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide KLH conjugated around amino acids 864~868 (E-D-SP-A-Y) derived from Human NFkB-p100/p52
Specificity This antibody detects endogenous levels of NF-κB p100/p52 protein around serine 865.
Formulation PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State: Aff - Purified
State: Liquid purified IgG fraction
Concentration lot specific
Purification Affinity Chromatography
Conjugation Unconjugated
Storage Condition Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Gene Name nuclear factor kappa B subunit 2
Background NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
Synonyms LYT10, KBF2, H2TF1, NF-kB p100, NF-kB p52, NF kappa B
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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