RPS6 pSer235 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).
Specificity	This antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at Serine 235.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	ribosomal protein S6
Database Link	Entrez Gene 20104 MouseEntrez Gene 29304 RatEntrez Gene 6194 Human UniProt ID P62753
Synonyms	Phosphoprotein NP33, 40S ribosomal protein S6
Reference Data
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway, Ribosome

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