MEK4 (MAP2K4) pThr261 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of threonine261 (A-K-Tp-R-D)
Specificity	AP02453PU antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at Threonine 261.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase kinase 4
Database Link	Entrez Gene 6416 Human UniProt ID P45985
Background	MEK4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8 / JNK1, MAPK9 / JNK2, and MAPK14 / p38, but not MAPK1 / ERK2 or MAPK3 / ERK3. This kinase is phosphorylated, and thus activated by MAP3K1 / MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Synonyms	JNK-activating kinase 1, MAP Kinase Kinase 4, SAPK/ERK kinase 1, PRKMK4, SERK1, MEK-4, MAPKK4, MKK4
Reference Data

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