CDK2 pThr160 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Suitable for use in Western blot (1:500~1:1000), ELISA (1:4000) and Immunohistochemistry (1:50~1:100).
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of threonine 160 (T-Y-TP-H-E).
Specificity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. CDK2 (phospho-Thr160) antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase 2
Database Link	Entrez Gene 1017 Human UniProt ID P24941
Background	Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Synonyms	p33 protein kinase
Reference Data

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