p53 (TP53) pSer315 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50~1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of Serine 315 (S-S-SP-P-Q).
Specificity	This antibody detects endogenous levels of p53 only when phosphorylated at Serine 315.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data

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