GSK3 beta (GSK3B) pSer9 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Note: Incubate membrane with diluted antibody in 5% nonfat milk, 1xTBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide Sequence around the phosphorylation site of Serine 9 (T-T-Sp-F-A) derived from Human GSK3B.
Specificity	This antibody detects endogenous levels of GSK3β only when phosphorylated at Serine 9.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibody was removed by chromatography using non-phosphopeptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	glycogen synthase kinase 3 beta
Database Link	Entrez Gene 2932 Human UniProt ID P49841
Background	Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. GSK3 has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control and oncogenesis. Two isoforms, alpha (GSK3A; OMIM 606784) and beta, show a high degree of amino acid homology within their catalytic domains. GSK3B is involved in energy metabolism, neuronal cell development and body pattern formation.
Synonyms	GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B
Note	Molecular Weight: 46 kDa
Reference Data

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