Ezrin (EZR) pTyr353 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1/500-1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of Ezrin pTyr353 protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 69 kDa
Gene Name	ezrin
Database Link	Entrez Gene 7430 Human UniProt ID P15311
Background	The microvillar core protein Ezrin is a tyrosine-phosphorylated protein foundin epidermal growth factor-stimulated A-431 carcinoma cells. Ezrin is phosphorylated transiently to a high level on Tyrosine residues 146 and 354. One of the sites, Tyrosine 146, lies in the amino-terminal region of homology that is common to the protein 4.1-Talin-Ezrin protein family. This tyrosine residue and its vicinal amino acids are conserved throughout the family which includes Radixin, Moesin and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG. Another phosphorylation site is Tyrosine 354, which is localized within the α-helical domain of Ezrin. Cytoplasmic signaling may result in activation of Ezrin in tyrosine phosphorylation and this suggests that Ezrin has qualities that might play a role in modulation of cell shape and adhesion.
Synonyms	p81, Cytovillin, Villin-2, EZR, VIL2
Reference Data

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