PAK4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1/500-1/1000.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	PAK4/5/6 antibody detects endogenous levels of PAK4/5/6 protein. (region surrounding Val469)
Formulation	Phosphate buffered saline (PBS) with 0.05% Sodium Azide as preservative, approx. pH 7.2. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 80 kDa
Gene Name	p21 (RAC1) activated kinase 4
Database Link	Entrez Gene 70584 MouseEntrez Gene 292756 RatEntrez Gene 10298 Human UniProt ID O96013
Background	Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.
Synonyms	PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, p21-activated kinase 7, PAK-7, p21-activated kinase 5, PAK-5, KIAA1264 , p21-activated kinase 6, PAK-6
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway

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