CCN5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: Direct: To detect hCTGFL/WISP-2 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Sandwich: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human CTGFL/WISP-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98 %) recombinant human CTGFL/WISP-2
Specificity	This antibody detects Connective Tissue Growth Factor-Like protein.
Formulation	PBS, pH 7.2 Label: Biotin State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
Purification	Affinity chromatography
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
Gene Name	WNT1 inducible signaling pathway protein 2
Database Link	Entrez Gene 8839 Human UniProt ID O76076
Synonyms	WISP-2, CCN5, CT58, CTGFL, CTGF-L, UNQ228/PRO261
Reference Data

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