BMP7 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP7 (100 ng/ml), a concentration of 0.40-0.60 µg/ml of this BMP7 antibody is required. Sandwich ELISA: To detect Human BMP7 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml is required. The antigen affinity purified BMP7 antibody, in conjunction with Biotinylated antibody anti-Human BMP7 antibody (AP01113BT-N or AP01113BT-S) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BMP-7. Western Blot: To detect Humnan BMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Hunan BMP-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	CHO cell derived recombinant highly pure (>98%) Human BMP-7.
Specificity	Detects BMP7.
Formulation	PBS, pH 7.2 without preservatives. State: Purified State: Lyophilized (Sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography using an immobilized Human BMP7 matrix.
Conjugation	Unconjugated
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
Gene Name	bone morphogenetic protein 7
Database Link	Entrez Gene 655 Human UniProt ID P18075
Background	Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.
Synonyms	BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1
Note	Centrifuge vial prior to opening!
Reference Data

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