IGFBP7 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	Sandwich ELISA: To detect Human IGF-BP7 (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human IGF-BP7 (Cat.-No AP01109BT-N or AP01109BT-S) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-BP7. Western Blot: To detect Human IGF-BP7 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IGF-BP7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.  Immunostaining: This antibody stained HeLa and U-251 MG cells. The primary antibody was incubated at 2.0 µg/ml overnight at 4°C followed by a Fluorescent labeled secondary antibody.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived recombinant Human IGFBP7 (Cat.-No AR09501PU).
Specificity	This antibody detects Human IGFBP7.  Other species not tesed.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Human IGF-BP7 matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	insulin like growth factor binding protein 7
Database Link	Entrez Gene 3490 Human UniProt ID Q16270
Background	IGF-BPs form high affinity complexes with both IGF-I and IGF-II and act to control of the distribution, function and activity of IGFs in various cell tissues and body fluids. There are seven named IGF-BPs. IGFBP7 plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. Additionally, IGFBP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle, and increased apoptosis. IGFBP7 is expressed in a wide range of normal human tissues and it usually shows reduced expression in cancer cell lines of prostate, breast, colon, and lung origin.
Synonyms	IGF-binding protein 7, IGFBP-7, IBP7, IBP-7, IGFBP-rP1, MAC25, PSF, TAF
Note	Centrifuge vial prior to opening.
Reference Data

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