Nrcam (30-845, Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: S364-51]

Specifications

Product Data
Clone Name	S364-51
Applications	IF, WB
Recommend Dilution	Western blot: 1/1000; 1μg/ml was sufficient for detection of NRCAMin 20 μg of rat brain lysate by colorimetric immunoblot using HRP conjugated secondary antibody. Immunocytochemistry.
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein amino acids 30-845 (extracellular domain) of mouse NRCAM protein.
Specificity	This antibody detects a 160 kDa protein. (~50% identity with Neurofascin)
Formulation	PBS pH 7.4, 50% Glycerol, 0,09% Sodium azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	neuronal cell adhesion molecule
Database Link	Entrez Gene 319504 Mouse UniProt ID Q810U4
Background	Neuronal cell adhesion molecule (NRCAM) is a cell surface protein of the immunoglobulin (Ig) superfamily. NRCAM (also known as Bravo) contains six Ig domains, five fibronectin repeats, a transmembrane region and an intracellular domain. NRCAM is expressed in brain, spinal cord, peripheral nervous system and pancreas. In the spinal cord, NRCAM acts as a ligand for axonin-1 to guide commissural axons across the floor plate. NRCAM also acts as a ligand for F3 to control actin-dependent growth cone motility. NRCAM interacts with neurofascin and may facilitate the clustering of the cystoskeletal protein ankyrin G and the voltage-dependent sodium channel proteins at the node of Ranvier. NRCAM expression may play a role in the severity of certain types of tumors. NRCAM is overexpressed in high-grade astrocytomas, gliomas and glioblastoma tumor tissues. In the pancreas, NRCAM expression is upregulated in intraductal hyperplasia. Antisense NRCAM reduces the tumorigenic properties of human glioblastoma cells in vitro and slowed tumor growth in vivo. The gene encoding human NRCAM maps to chromosome 7q31.1-q31.2.
Synonyms	Nr-CAM, Neuronal cell adhesion molecule, Bravo, KIAA0343
Reference Data

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