PAX6 Mouse Monoclonal Antibody [Clone ID: PAX6/496]

Specifications

Product Data
Clone Name	PAX6/496
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5 µg/ml. Western Blotting: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Positive Control: Eye or Cerebellum.
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human PAX6 protein.
Specificity	Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters’ anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain. Cellular Localization: Nuclear.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromnatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	47 kDa
Gene Name	paired box 6
Database Link	Entrez Gene 5080 Human UniProt ID P26367
Background	Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters’ anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.
Synonyms	Pax-6, Aniridia type II protein, AN2, Oculorhombin
Reference Data

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