MelanA (MLANA) Mouse Monoclonal Antibody [Clone ID: M2-7C10 + M2-9E3 + T311 + HMB45]
CAT#: AM50296PU-S
MelanA (MLANA) mouse monoclonal antibody, clone M2-7C10 + M2-9E3 + T311 + HMB45, Purified
Size: 20 ug
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CNY 5,606.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | M2-7C10 + M2-9E3 + T311 + HMB45 |
Applications | IHC |
Recommend Dilution | Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Recombinant Human MART-1 protein (M2-7C10; M2-9E3); Recombinant Tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45). Isotypes: Mouse/IgG2b (M2-7C10 & M2-9E3) + Mouse/IgG2a (T311) + Mouse/IgG1 (HMB45) |
Specificity | This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Cellular Localization: Cytoplasmic. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | lot specific |
Purification | Protein A/G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. |
Predicted Protein Size | 20-22 kDa (doublet) (MART); 70-80 kDa (Tyrosinase); 90-100 kDa (gp100) |
Gene Name | melan-A |
Database Link | |
Synonyms | Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17 |
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