HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: HIF1A-84]

Specifications

Product Data
Clone Name	HIF1A-84
Applications	FC, IF
Recommend Dilution	ELISA: For coating, order Ab without BSA. Flow Cytometry: 0.5-1 µg/million cells.  Immunofluorescence: 0.5-1 µg/ml. Functional Studies: Use Antibody without BSA and Azide. Positive Control: Cobalt chloride treated HeLa cells. Breast or bladder carcinomas.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human HIF1 alpha protein.
Specificity	This Antibody reognizes HIF1 alpha. Cellular Localization: Nuclear and cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	92-110 kDa
Gene Name	hypoxia inducible factor 1 alpha subunit
Database Link	Entrez Gene 3091 Human UniProt ID Q16665
Background	HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Synonyms	HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78
Reference Data

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