hCG beta (CGB3) Mouse Monoclonal Antibody [Clone ID: HCGb/459]

Specifications

Product Data
Clone Name	HCGb/459
Applications	IHC, WB
Recommend Dilution	ELISA: For coating, order Ab without BSA. Western Blot: 0.5-1 µg/ml. Immunohistochemsitry on Frozen and formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Placenta.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant hCG beta protein.
Specificity	This Monoclonal Antibody reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior. Cellular Localization: Cytoplasmic, secreted.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	22 kDa
Gene Name	chorionic gonadotropin beta subunit 3
Database Link	Entrez Gene 1082 Human UniProt ID P0DN86
Synonyms	Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta
Reference Data

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