Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: A53-B/A2.26 + BA17]

Specifications

Product Data
Clone Name	A53-B/A2.26 + BA17
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: Not tested (0.5-1 μg/ml). Immunoprecipitation: Not tested (1-2 μg/500 μg protein lysate). Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: MCF-7, HeLa cells, Breast Cancer.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Human breast cancer MCF-7 cells (A53-B/A2); Human mammary epithelial organoids (BA17).
Specificity	This Monoclonal Antibody reacts with the rod domain of Human Cytokeratin 19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	40 kDa
Gene Name	keratin 19
Database Link	Entrez Gene 3880 Human UniProt ID P08727
Synonyms	Cytokeratin-19, Keratin-19, KRT19, CK19, K19
Reference Data

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