ICAM3 Mouse Monoclonal Antibody [Clone ID: 186-2G9]

Specifications

Product Data
Clone Name	186-2G9
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Functional Studies: Blocks binding of CD11a (LFA-1) to CD50 (Use Azide free Antibody). ELISA: Use BSA free Antibody for Coating. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Stimulated Human leukocytes.
Specificity	Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This Monoclonal Antibopdy is excellent for staining of formalin/paraffin tissues. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	110-160 kDa
Gene Name	intercellular adhesion molecule 3
Database Link	Entrez Gene 3385 Human UniProt ID P32942
Synonyms	ICAM-3, ICAM-R, CDw50
Reference Data

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