CD28 Mouse Monoclonal Antibody [Clone ID: C28/77]

Specifications

Product Data
Clone Name	C28/77
Applications	FC, IF, IHC, IP
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Functional Studies: Use Antibody without BSA and Azide. Immunoprecipitation: 0.5-1 µg/500ug protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Jurkat cells or human lymph nodes and tonsils.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human CD28 protein.
Specificity	Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader. Cellular Localization: Cell surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	44 kDa (monomer); 88 kDa (dimer)
Gene Name	CD28 molecule
Database Link	Entrez Gene 940 Human UniProt ID P10747
Synonyms	TP44
Reference Data

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