CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: B9E9]

Specifications

Product Data
Clone Name	B9E9
Applications	IF, IHC, IP
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Functional Studies: (Use Antibody without BSA and Azide. Flow Cytometry: 0.5-1 µg/million cells. Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 μg/500 µg protein lysate. Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 min at RT. Positive Control: Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Lymphoblastoid cell line Daudi.
Specificity	Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This Monoclonal Antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. Cellular Localization: Predominantly cell surface with some cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	33-37 kDa
Gene Name	membrane spanning 4-domains A1
Database Link	Entrez Gene 931 Human UniProt ID P11836
Background	CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Synonyms	MS4A1, Leu-16, Bp35, B-cell marker
Reference Data

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