PGP9.5 (UCHL1) Mouse Monoclonal Antibody [Clone ID: SPM574]

Specifications

Product Data
Clone Name	SPM574
Applications	IHC, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Western Blotting: 0.5-1 µg/ml. Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Cerebellum.
Reactivity	Bovine, Human, Mouse, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Native UchL1 (PGP9.5) protein from brain.
Specificity	This Monoclonal Antibody reacts with a protein of 20-30kDa, identified as PGP9.5, also known as Uubiquitin carboxyl-terminal hydrolase-1 (UchL1). Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	20-30 kDa
Gene Name	ubiquitin C-terminal hydrolase L1
Database Link	Entrez Gene 7345 Human UniProt ID P09936
Background	PGP9.5, also known as Uubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Synonyms	UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5
Reference Data

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