Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: SPM286]

Specifications

Product Data
Clone Name	SPM286
Applications	FC, IF, IHC
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Immunohistochemistry on Frozen and Formalin-fixed Sections: 1-2 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: SK-MEL-28 cells or Melanoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Membranes from a human melanoma metastasis.
Specificity	By Immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	90-100 kDa
Gene Name	premelanosome protein
Database Link	Entrez Gene 6490 Human UniProt ID P40967
Synonyms	SILV, D12S53E, PMEL17, ME20-M
Reference Data

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