MYOD1 Mouse Monoclonal Antibody [Clone ID: SPM427]

Specifications

Product Data
Clone Name	SPM427
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/ml. Western Blotting: 0.25-0.5 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 μg/ml for 30 minutes at RT.  Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Rhabdomyosarcoma.
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant mouse MyoD1 protein.
Specificity	Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this Monoclonal Antibody maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with Myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas. Cellular Localization: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	45 kDa
Gene Name	myogenic differentiation 1
Database Link	Entrez Gene 4654 Human UniProt ID P15172
Synonyms	MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3, BHLHC1
Reference Data

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