alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: SPM334]

Specifications

Product Data
Clone Name	SPM334
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Reactivity	Canine, Human, Monkey, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Alpha fetoprotein (AFP) purified from serum of a hepatoma patient.
Specificity	It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This Monoclonal Antibody is is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Cellular Localization: Cytoplasmic. Negative Species: Cow, Cat, Mouse, and Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	70 kDa
Gene Name	alpha fetoprotein
Database Link	Entrez Gene 174 Human UniProt ID P02771
Background	Alpha-Feto Protein (AFP) is a protein produced by the fetus, which is excreted into the amniotic fluid and into the mother's bloodstream through the placenta. The amount of AFP, both in the maternal's blood and in the amniotic fluid, at particular periods during the pregnancy, may be associated with the presence of neural tube defects or chromosomal problems in the baby. AFP synthesis occurs in tissues including the gut, liver and fetal yolk sac. AFP expression may be seen in germ cell neoplasma and in liver carcinoma.
Synonyms	Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
Reference Data

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