IGF1 Mouse Monoclonal Antibody [Clone ID: M23]

Specifications

Product Data
Clone Name	M23
Applications	ELISA, FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 1-2 μg/ml. In-vitro Neutralization of Biological Activity of IGF-1 (Use Azide Free Antibody). Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein.
Reactivity	Human, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human IGF-1 protein.
Specificity	This antibody is specific to Insulin-like Growth Factor (IGF-I) and shows minimal cross-reaction with IGF-II, Proinsulin, MSF, and Insulin. Clone M23 is capable of inhibiting IGF-I activity in bioassays for insulin-like, mitogenic and sulfation activities. Cellular Localization: Cytoplasmic (Secreted).
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~7.6 kDa
Gene Name	insulin like growth factor 1
Database Link	Entrez Gene 3479 Human UniProt ID P05019
Background	Insulin-like growth factor 1 (IGF-1) is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon. IGF-1 is involved in regulation of neuronal growth and development in central and peripheral nervous system. It is known to protect neurons against cell death induced by amyloidogenic derivatives, glucose or serum deprivation through pathways involving AKT kinase and transcription factor FKHRL1 phosphorylation. Activation of the insulin-like growth factor system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like breast and thyroid cancers.
Synonyms	IGF-I, Somatomedin-C, Mechano growth factor, MGF, IBP1
Reference Data

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