Bromodeoxyuridine / BrDU Mouse Monoclonal Antibody [Clone ID: BRD494]

CAT#: AM33307PU-T

Bromodeoxyuridine / BrDU mouse monoclonal antibody, clone BRD494, Purified

Size: 20 ug 100 ug



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CNY 3,223.00


货期*
5周

规格
    • 20 ug

Product images

Specifications

Product Data
Clone Name BRD494
Applications FC, IF, IHC
Recommend Dilution Flow Cytometry: 0.5-1 µg/106 cells.
Immunofluorescence: 0.5-1 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed
Sections: 0.5-1 µg/ml for 30 minutes at RT.
For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37°C.
Recommended Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Reactivity All Species
Host Mouse
Clonality Monoclonal
Immunogen Bromodeoxyuridine (BrdU) conjugated to KLH
Specificity It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Cellular Localization: Nuclear.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
DO NOT FREEZE!
Predicted Protein Size Depends on the target
Background Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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