BCL10 (122-168) Mouse Monoclonal Antibody [Clone ID: SPM520]

Specifications

Product Data
Clone Name	SPM520
Applications	FC, IF, IHC, WB
Recommend Dilution	ELISA: Use BSA Free antibody for coating. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 1-2 µg/ml. Western Blotting: 0.5-1 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: WEHI-231 or Ramos cells or lymphoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human Bcl-10 protein (epitope aa122-168)
Specificity	This Monoclonal antibody labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	ProteinA/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	33 kDa
Gene Name	B-cell CLL/lymphoma 10
Database Link	Entrez Gene 8915 Human UniProt ID O95999
Background	BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NFκB activation in a NIK-dependent pathway. Bcl10 / CIPER/ CLAP / mE10 is expressed in many human and murine tissues. It contains a CARD (caspase recruiting domain) domain and is involved in activation of caspase9 to induce apoptosis. It is a cellular homolog of equine herpesvirus 2 E-10. It is known to induce activation of JNK, p38 and NF-kappaB. Mutations in Bcl10 are observed in many B and T cell lymphomas implicating its role in pathogenesis of human cancer.
Synonyms	BCL10, CIPER, CLAP, cCARMEN, mE10, c-E10, hCLAP
Reference Data

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