Melanoma gp100 (PMEL) (PMEL17 / SILV) Mouse Monoclonal Antibody [Clone ID: HMB45]

Specifications

Product Data
Clone Name	HMB45
Applications	FC, IF, IHC
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml. Immunofluorescence: 0.5-1 μg/ml. Immunohistochemistry on Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: SK-MEL-28 cells or Melanoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Extract of pigmented melanoma metastases from lymph nodes.
Specificity	By Immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This Monoclonal Antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Anti-HMB-45 stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This Monoclonal Antibody also stains Angiomyolipoma (PEComa). Cellular Localization: Cytoplasmic. Negative Species: Dog and Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A or G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	90-100 kDa
Gene Name	premelanosome protein
Database Link	Entrez Gene 6490 Human UniProt ID P40967
Synonyms	SILV, D12S53E, PMEL17, ME20-M
Note	Hu-chromosome location: 12q13.2 Theoretical MW: 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reference Data

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