S100A1 Mouse Monoclonal Antibody [Clone ID: 4C4.9]

CAT#: AM32830PU-S

S100A1 mouse monoclonal antibody, clone 4C4.9, Purified

Size: 20 ug 100 ug



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CNY 5,606.00


货期*
5周

规格
    • 100 ug

Cited in 1 publication.

Product images

Specifications

Product Data
Clone Name 4C4.9
Applications FC, IF, IHC, IP, WB
Recommend Dilution ELISA: Use Antibody without BSA for Coating.
Western Blot:
 
0.5-1 µg/ml. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate.
Flow Cytometry: 0.5-1 µg/106 cells.
Immunofluorescence:
1-2 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use C4.9 antibody at 0.25-0.5 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control: Brain, Melanoma.
Reactivity Bovine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen S100 protein purified from Bovine brain.
Specificity This Monoclonal antibody stains S100 protein.
4C4.9 stains Schwannomas, ependymomas, astrogliomas; almost all benign and malignant melanomas and their metastases.
Cellular Localization: Cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C.
Predicted Protein Size 10-12 kDa
Gene Name S100 calcium binding protein A1
Background S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas and their metastases.
Synonyms S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain, S-100 protein beta chain, Astrocyte Marker
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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