DHLAG (CD74) Mouse Monoclonal Antibody [Clone ID: LN2]

Specifications

Product Data
Clone Name	LN2
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: For coating, Use Antibody without BSA. Western Blot: 0.5-1 µg/ml. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 1-2 µg/ml. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Daudi Cells or Raji Cells. Tonsil or lymph node.
Reactivity	Human, Mouse, Baboon
Host	Mouse
Clonality	Monoclonal
Immunogen	SU-DHL-4 lymphoma cells.
Specificity	This monoclonal LN2 antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells. It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). Cellular Localization: Cell surface and paranuclear globular. Negative Species: Rat.
Formulation	10 mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	33-41 kDa
Gene Name	CD74 molecule
Database Link	Entrez Gene 972 Human UniProt ID P04233
Background	CD74, a Type-II transmembrane protein, is a unique member of RIP-processed protein family. It consists of an extracytoplasmic domain, a transmembrane segment and an intracytoplasmic domain. It is expressed in B-cells, monocytes, macrophages and other Class II MHC expressing cells. CD74 associates with alpha and beta chains of HLA-DR (MHC class II) and directs intracellular sorting of MHC class II molecules. It has a prominent role in antigen presentation and as an accessory signaling molecule. Cell-surface CD74 binds to MIF and its expression is required for MIF-mediated ERK1/2-phosphorylation, PGE2 production and cell proliferation. CD74 regulates B-cell differentiation and acts as a survival receptor through activation of NF-kB p65/RelA homodimer. Pathological role of CD74 has been identified in different cancers including Multiple Myeloma.
Synonyms	p33, DHLAG
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Antigen processing and presentation

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