Chromogranin A (CHGA) Mouse Monoclonal Antibody [Clone ID: PHE5]

Specifications

Product Data
Clone Name	PHE5
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for Coating. Western Blot: 0.5-1 µg/ml. Flow Cytometry: 0.5-1 µg/106 cells. Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 0.5-1 µg/500 µg protein. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Postive Control: PC12 cells, Adrenal gland, bowel, thyroid, pancreas, or pheochromocytoma.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Human phaeochromocytoma.
Specificity	Recognizes Human and Monkey Chromogranin A. Other species not tested. Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. Cellular Localization: Finely granular cytoplasmic. Negative Species: Mouse, Rat.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8 °C.
Predicted Protein Size	68-75 kDa
Gene Name	chromogranin A
Database Link	Entrez Gene 1113 Human UniProt ID P10645
Background	Chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Synonyms	Chromogranin-A, CgA, CHGA, SP-I
Reference Data

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