Integrin alpha 5 (ITGA5) Mouse Monoclonal Antibody [Clone ID: NKI-SAM1]

CAT#: AM32393PU-N

Integrin alpha 5 (ITGA5) mouse monoclonal antibody, clone NKI-SAM1, Purified



Need it in bulk or conjugated?
Get a free quote

CNY 3,384.00


货期*
5周

规格
    • 1 ml

Product images

Specifications

Product Data
Clone Name NKI-SAM1
Applications FC, IHC
Recommend Dilution Fluorescence experiments: NKI-SAM-1 is suitable for Flow Cytometry (using 0.5-1 µg for 106 cells) and can be used to determine the VLA-5 expression in peripheral blood monocytes and monocyte derived dendritic cells B cells and B-cell precursors.
Immunohistochemistry on Frozen Sections: 1/20 (Acetone Fixation).
Reactivity Human
Host Mouse
Clonality Monoclonal
Specificity This antibody recognizes a 150/130 kD receptor for fibronectin, (VLA-alpha-5 protein from the integrin superfamily of antigens).
It reacts with platelets, lymphocytes, monocytes and most cell lines including K562 and U937.
Formulation PBS
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Conjugation Unconjugated
Storage Condition Store the antibody undiluted at 2-8°C.
Gene Name integrin subunit alpha 5
Background Integrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their environment, being both other cells or the extra- cellular matrix. Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated subunits, i.e. an α-subunit of 95 kDa that is conserved through the superfamily and a more variable β subunit of 150-170 kDa. More than 18 α and 8 β subunits with numerous splice variant isoforms have been identified in mammals. The integrin α5 chain (160 kDa) (CD49e), undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light (25 kDa) and heavy (135 kDa) chains, which non-covalently associate with the integrin β1 subunit (CD29) (130 kDa), thus forming the heterodimer α5-β1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor (FNr) that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5 mediates binding of T- and B-cells to fibronectin, an extracellular matrix glycoprotein involved in cell adhesion and migration in wound healing, embryonic development and malignant transformation. The VLA-5 or FNr binds only fibronectin recognizing the Arg-Gly-Asp (RGD) sequence in the central region of the molecule.
Synonyms Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, VLA5, FNRA
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...