Lipid A Mouse Monoclonal Antibody [Clone ID: 26-5]

Specifications

Product Data
Clone Name	26-5
Applications	AGG, ELISA
Recommend Dilution	ELISA. Agglutination assays. Recommended Dilutions: For ELISA use a dilution of 1/10 in PBS containing 4% BSA and 0.05% Tween. For Agglutination Tests: The antibody should be used undiluted. For ELISA prepare bacteria suspension in PBS (5x108 bact. /ml). Coat ELISA plate with 100 μl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tap water with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-Mouse Ig Enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.
Reactivity	Gram Negative Bacteria
Host	Mouse
Clonality	Monoclonal
Specificity	This Monoclonal antibody is reactive with Lipid A using ELISA.
Formulation	PBS State: Purified State: Liquid (0.2 µm filtered) purified Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	Lipid A is part of a Gram-negative bacterial endotoxin located at one end of the lipopolysaccharide (LPS) molecule. Lipid A is made up of two glucosamine units with attached acyl chains, and it usually contains one phosphate group on each carbohydrate. Lipid A functions to anchor the LPS to the outer membrane of a Gram-negative bacteria. The toxicity of Gram-negative bacteria is due to Lipid A since this is what the human immune system recognizes, though this recognition is also critical for the onset of immune responses to Gram-negative infection and for the subsequent successful fight against the infection. Lipid A may play a role in to activating cells via Toll-like receptor 4 (TLR4), MD-2, and CD14 on the cell surface. When present in the body at high concentrations during a Gram-negative bacterial infection, Lipid A can cause shock and death because it is such a potent immune system activator.
Reference Data

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