CD21 (CR2) Mouse Monoclonal Antibody [Clone ID: 2G9]

Specifications

Product Data
Clone Name	2G9
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections. Recommended Dilutions: 1/25-1/100 Recommended Positive Control: Tonsil, Lymph Node. Preparation and Pretreatment 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises the Human CD21 cell surface antigen, a 140 glycoprotein. CD21 is expressed by mature B lymphocytes. Anti-CD21 is useful in the identification of follicular dendriditic cell matrix found in normal lymph nodes and tonsillar tissue. This antibody also labels follicular dendritic cell tumor/sarcomas. The antigen is absent on T-lymphocytes, monocytes, and granulocytes.
Formulation	Tris Buffer, pH 7.3-7.7 State: Supernatant State: Liquid Tissue Culture Supernatant Stabilizer: 0.9% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement component 3d receptor 2
Database Link	Entrez Gene 1380 Human UniProt ID P20023
Background	CD21, also known as CR2, C3d receptor and EBV receptor, is expressed strongly on mature B cells, follicular dentritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.
Synonyms	Complement receptor type 2, CR2, C3DR, C3d receptor, EBV Receptor, Dendritic Cell Marker
Reference Data

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