CD62E (SELE) Mouse Monoclonal Antibody [Clone ID: ENA1]

Specifications

Product Data
Clone Name	ENA1
Applications	IHC
Recommend Dilution	Immunohistochemistry on frozen sections. The typical starting working dilution is 1/10.  In vitro cultured cells can be fixed with 1% paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-mouse Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 μg/ml) in tissue culture medium at 37°C. As negative control it is advised to use a control mouse IgG1 antibody. Immunoprecipitation. Immunoassay.  For adhesion inhibition studies we advise to use the F(ab’)2 ENA2 monoclonal antibody preparation (Cat. nr. AM32174PU-N), which is most efficient for this purpose and prevents adhesion of leucocytes via Fc receptors.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	ENA1 reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). The antibody reacts with human endothelial cells activated with TNF-alpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Formulation	PBS State: Liquid State: Liquid purified Ig fraction Stabilizer: 0,1% BSA Preservative: 0.02% sodium azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	selectin E
Database Link	Entrez Gene 6401 Human UniProt ID P16581
Synonyms	SELE, ELAM1, LECAM2
Reference Data

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