HLAB (HLA-B) Mouse Monoclonal Antibody [Clone ID: EP-4]

Specifications

Product Data
Clone Name	EP-4
Applications	IHC
Recommend Dilution	Immunohistochemistry on Paraffin Sections: For use with paraformaldehyde fixed cell suspensions or cytospins. Recommended Positive Control Tissue: Blood.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Native purified from lymphocytes of HLA-B27 individual.
Specificity	This antibody clone EP-4 recognizes the HLA-B27 cell surface antigen on Human cells. Since EP-4 recognizes the HLA-B27 antigen, it may be used to HLA type human lymphoctyes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in Human lymphocytes. Monoclonal antibody EP-4 recognizes the HLA-B27 antigen expressed on individuals with this HLA haplotype.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	major histocompatibility complex, class I, B
Database Link	Entrez Gene 3106 Human UniProt ID P01889
Background	HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described
Synonyms	HLAB, MHC class I antigen B7
Reference Data

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