ABCC10 Rat Monoclonal Antibody [Clone ID: M7I-3]

Specifications

Product Data
Clone Name	M7I-3
Applications	IF, IHC, WB
Recommend Dilution	Western blotting: Use at 1/20-1/50 and anti-Rat-HRP. Immunocytochemistry: Use at 1/20-1/50 on Acetone Fixed Cytospin preparations. Immunohistochemisty on Frozen Sections: 1/20 on Acetone Fixed Frozen sections can be followed by incubation with Rabbit anti-Rat Ig (1/25) and a monoclonal Rat APAAP complex (1/50). Alternatively, after incubation with M7I-3 (use 1/20) and washing, slides can be incubated with biotinylated rabbit anti-Rat Ig (1/100) and streptavidin conjugated to horseradish peroxidase (1/500).
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	A bacterial fusion protein of Human MRP7, containing amino acids 194-272 of the protein.
Specificity	This Monoclonal antibody M7I-3 reacts with an internal epitope of MRP7 (ABCC10), an approximately 160 kD transmembrane protein that is related to the multidrug resistance protein MRP1. M7I-3 has potential value for the detection of MRP7-mediated drug-resistance in Human tumor samples.
Formulation	State: Supernatant State: Serum Free Culture Supernatant Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store antibody undiluted at 2-8°C.
Gene Name	ATP binding cassette subfamily C member 10
Database Link	Entrez Gene 89845 Human UniProt ID Q5T3U5
Background	The MRP family is represented by nine similar ABC transporters that have the ability to transport structurally diverse lipophilic anions and operate as chemical efflux pumps. MRP7 (multidrug resistance-associated protein 7, ATPbinding cassette sub-family C member 10) is a multi-pass membrane protein that belongs to the ABC transporter family (conjugate transporter subfamily). MRP7 is involved with the ATP-dependent transport of 17β-estradiol-D-17-β-glucuronide (E217βG). MRP7 is also probably involved in cellular detoxification through its lipophilic anion extrusion capabilities. MRP7 contains two ABC transmembrane type 1 domains and two ABC transporter domains. MRP7 likely has three isoforms. Isoform 2 is the most widely expressed, while isoform 1 is predominately expressed in the spleen.
Synonyms	Multidrug resistance-associated protein 7, SIMRP7
Note	1 ml vials >> 200 tests
Reference Data

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