CD45 / LCA (CD45RC) Rat Monoclonal Antibody [Clone ID: IBL-8]

Specifications

Product Data
Clone Name	IBL-8
Applications	FC, IHC, IP
Recommend Dilution	Immunohistochemistry on acetone-fixed frozen sections. Flow Cytometry. Immunoprecipitation.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody reacts to CD45.
Formulation	PBS containing 0.02% sodium azide (NaN3) as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	CD45 (L-CA) is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. This purified anti-mouse CD45 RC is directed against the exon C-dependent RC isoform and reacts strongly with B cells, and less intensely with most CD8+ T cells. It does not recognize CD4+ T cells and myeloid cells do not express the RC isoform.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 2.0 μg of this antibody per 10e6 cells.. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 μl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at 1/500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 μl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 2.0μg/10e6 cells Isotypic Control: Purified Rat IgG1
Reference Data

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