Chlamydia (LPS) Mouse Monoclonal Antibody [Clone ID: B352M]

Specifications

Product Data
Clone Name	B352M
Applications	ELISA
Recommend Dilution	ELISA: Use this antibody AM31415PU-N (Clone B352M) as Capture and AM31413PU-N (Clone B351M) as Detection antibody.
Reactivity	Chlamydia pneumoniae
Host	Mouse
Clonality	Monoclonal
Immunogen	Chlamydia trachomatis elementary bodies. Source: Ascites
Specificity	Specific to LPS of Chlamydia. Genus specific. Reactive with C. trachomatis, C. pneumoniae and C. psittaci.
Formulation	0.01M PBS, pH 7.2 containing 0.09% Sodium Azide as preservative and no stabilizing proteins State: Purified State: Liquid purified IgG fraction (> 90% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Background	Chlamydia is caused by the bacterium Chlamydia trachomatis. The intracytoplasmic inclusions caused by the bacterium are draped around the infected cell's nucleus. Chlamydia trachomatis is an intracellular organism that has a genome size of approximately 500-1000 kilobases and contains both RNA and DNA. The organism is also extremely temperature sensitive and must be refrigerated at 4°C as soon as a sample is obtained. Colonization of Chlamydia begins with attachment to sialic acid receptors on the eye, throat or genitalia. It persists at body sites that are inaccessible to phagocytes, T cells, and B cells. It also exists as 15 different serotypes. These serotypes cause four major diseases in humans: endemic trachoma (caused by serotypes A and C), sexually transmitted disease and inclusion conjunctivitis (caused by serotypes D and K), and lymphogranuloma venereum (caused by serotypes L1, L2, and L3). Studies reveal that Chlamydia, because of its cell wall, is able to inhibit phagolysosome fusion in phagocytes. The cell wall is proposed to be Gram negative in that it contains an outer lipopolysaccharide membrane, but it lacks peptidoglycan in its cell wall. This lack of peptidoglycan is shown by the inability to detect muramic acid and antibodies directed against it. It may, however, contain a carboxylated sugar other than muramic acid. The proposed structure consists of a major outer membrane protein cross linked with disulfide bonds. It also contains cysteine rich proteins (CRP) that may be the functional equivalent to peptidoglycan. This unique structure allows for intracellular division and extracellular survival (Hatch 1996). Chlamydia usually infects the cervix and fallopian tubes of women and the urethra of men. Chlamydial infections are believed to be one of the most common of all STDs. It is generally thought that in a population of 15 million, there are up to 300,000 cases of chlamydia each year. Thus, there are many undiagnosed cases of chlamydia in the community. It has been estimated that the true prevalence of chlamydia in the sexually active population may be in the order of 5% to 10%. Chlamydia is one of the leading causes of blindness in underdeveloped countries.
Reference Data

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