Cd247 Tyr153 Mouse Monoclonal Antibody [Clone ID: EM-17]

Specifications

Product Data
Clone Name	EM-17
Applications	FC, WB
Recommend Dilution	Flow cytometry: 2-10 µg/ml. Positive control: Jurkat cells treated with pervanadate. Western blot: 2-5 µg/ml (non-reducing conditions). Positive control: Jurkat cells lysate treated with pervanadate; Splenocyte lysate of Balb/c or F1 mouse treated with pervanadate.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH
Specificity	This antibody recognizes phosphorylated tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Formulation	Phosphate buffered saline (PBS) State: Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide, approx. pH 7.4
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD247 antigen
Database Link	Entrez Gene 12503 Mouse UniProt ID P24161
Background	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Synonyms	T3Z, TCRZ, CD3 zeta chain
Reference Data

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