Piccolo (PCLO) Mouse Monoclonal Antibody [Clone ID: PCLO-01]

Specifications

Product Data
Clone Name	PCLO-01
Applications	FC, WB
Recommend Dilution	Western Blot: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAGE. Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant PCLO protein
Specificity	This antibody recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytomatrix of the neurons.
Formulation	Phosphate buffered saline (PBS), approx. pH 7.4 State: Aff - Purified State: Liquid ig fraction Preservative: 15 mM sodium azide
Concentration	lot specific
Purification	Protein-A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	piccolo presynaptic cytomatrix protein
Database Link	Entrez Gene 27445 Human UniProt ID Q9Y6V0
Background	PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.
Synonyms	Protein piccolo, Aczonin, ACZ, KIAA0559
Reference Data

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