CD5 Mouse Monoclonal Antibody [Clone ID: L17F12]

Specifications

Product Data
Clone Name	L17F12
Applications	FC, IF, IP, WB
Recommend Dilution	Flow cytometry: 1-4 μg/ml. Immunoprecipitation. Western blot: Laurylmaltoside lysing buffer; non-reducing conditions; 1-2 μg/ml. Immunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human acute lymphoblastic leukemia (ALL) T cells
Specificity	The antibody reacts with CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Formulation	Phosphate buffered saline (PBS), approx. pH 7.4 State: Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD5 molecule
Database Link	Entrez Gene 921 Human UniProt ID P06127
Background	CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Synonyms	CD5, LEU1
Reference Data

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