HLA DM (HLA-DMA) Rat Monoclonal Antibody [Clone ID: M5/114]

Specifications

Product Data
Clone Name	M5/114
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 2 μg/ml. Immunoprecipitation. Western blot. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Functional Application: Blocking of T cell proliferative responses.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Activated C57BL/6 mouse spleen cells
Specificity	The antibody reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Formulation	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized State: Azide Free State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein-G Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	major histocompatibility complex, class II, DM alpha
Database Link	UniProt ID P28067
Background	MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Synonyms	DMA, RING6, MHC class II antigen DMA
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.