Mpo Mouse Monoclonal Antibody [Clone ID: 8F4]

Specifications

Product Data
Clone Name	8F4
Applications	ELISA, IHC
Recommend Dilution	Immunohistochemistry on frozen sections (3,4): The typical starting working dilution is 1:50. Flow cytometry (2,5): The typical starting working dilution is 1:50. Immunoassays (1,3). Positive control: Neutrophils isolated from digested infarcts. Negative control: Lymphocytes isolated from digested infarcts. Does not work in Immunohistochemistry on paraffin sections.
Reactivity	Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified mouse MPO from WEHI-3 cells
Specificity	The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO).
Formulation	PBS Label: Biotin State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Biotin
Storage Condition	Store at 2 - 8 °C.
Gene Name	myeloperoxidase
Database Link	Entrez Gene 17523 Mouse UniProt ID P11247
Background	MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa. MPO is stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms. Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an diagnostic marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.
Synonyms	MPO
Reference Data

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